Researchers found that women who had worked night shifts (including those who had done so for up to 30 years) had no increased risk of breast cancer compared with women who had never worked shifts.

In the Million Women Study, EPIC-Oxford, and UK Biobank respectively, 673, 28 and 67 women who reported night shift work developed breast cancer. The combined relative risks taking all ten studies together were 0.99 for any night shift work, 1.01 for 20 or more years of night shift work, and 1.00 for 30 or more years night shift work.

The findings contradict a 2007 review by the International Agency for Research on Cancer that said shift work disrupted the “body clock” and was a probable cause of cancer. This was mainly based on animal and lab studies.

The latest work builds on a Canadian study of 1,134 breast cancer sufferers and 1,179 women of similar ages without the disease which found the risk factor was apparent only in those working nights for more than half a normal working life.

Dr Ruth Travis, lead author and a Cancer Research UK-funded scientist at the Cancer Epidemiology Unit at the University of Oxford, said: “We found that women who had worked night shifts, including long-term night shifts, were not more likely to develop breast cancer, either in the three new UK studies or when we combined results from all ten studies that had published relevant data.”

The research, Night Shift Work and Breast Cancer Incidence: Three Prospective Studies and Meta-analysis of Published Studies, was funded by the HSE, Cancer Research UK and the UK Medical Research Council.

Sarah Williams, Cancer Research UK’s health information manager, said: “This study is the largest of its kind and has found no link between breast cancer and working night shifts. We hope [this] news reassures women who work night shifts.”

Professor Andrew Curran, chief scientific adviser for the HSE, which commissioned the study, added: “This study has shown that night shift work, including long-term shift work, has little or no effect on breast cancer incidence in women. However, there are a number of other known risks with shift work that employers must take into consideration when protecting their workers’ health and safety.”

On average, one in seven (14%) women in the UK have ever worked nights and one in 50 (2%) have worked nights for 20 or more years. Each year in the UK around 53,300 women are diagnosed with breast cancer and around 11,500 die from the disease.